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4M7M

Crystal structure of the R111K:R132Q:Y134F:T54V:R59W:A32W mutant of the Cellular Retinoic Acid Binding Protein Type II in complex with All-Trans Retinal at 2.57 Angstrom Resolution

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-D
Synchrotron siteAPS
Beamline21-ID-D
Temperature [K]100
Detector technologyCCD
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)1.0782
Spacegroup nameP 31 2 1
Unit cell lengths56.457, 56.457, 109.775
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution44.663 - 2.571
R-factor0.2054
Rwork0.203
R-free0.25260
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.334
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwarePHENIX ((phenix.refine: 1.7.2_869))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.893
High resolution limit [Å]2.5282.528
Number of reflections7165
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP72770.1 M malonate, pH 7.0, 12% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K

217705

數據於2024-03-27公開中

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