4JZJ
Crystal Structure of Receptor-Fab Complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 88.300, 120.648, 92.968 |
| 格子定数 [度] | 90.00, 97.45, 90.00 |
精密化法
| 残基 | 49.675 - 2.801 |
| R因子 | 0.1878 |
| Rwork | 0.185 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.456 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (dev_1218) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.850 |
| 分解能 [Å] (高) | 2.800 | 2.800 |
| 独立反射数 | 47194 | |
| <I/σ(I)> | 25 | |
| 完全性 [%] | 99.4 | 99.9 |
| 冗長性 | 3.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 22% PEG 3350, 200mM Sodium Malonate, 100mM Citrate Buffer, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
