4JYP
crystal Structure of KAI2 Apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 50.690, 53.210, 55.770 |
Unit cell angles | 89.49, 89.76, 63.84 |
Refinement procedure
Resolution | 26.488 - 1.300 |
R-factor | 0.1699 |
Rwork | 0.169 |
R-free | 0.19060 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.091 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER (2.4.0) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 26.488 |
High resolution limit [Å] | 1.300 |
Rmeas | 0.109 |
Rpim | 0.064 |
Total number of observations | 261965 |
Number of reflections | 115956 |
<I/σ(I)> | 6.7 |
Completeness [%] | 90.1 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 22% (w/v) polyethylene glycol 8000, 0.1 M Na+-PIPES (pH 6.5), 0.3 M NaBr and 2 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298K |