4JA7
Rat PP5 co-crystallized with P5SA-2
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-28 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 51.100, 51.100, 365.670 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 15.000 - 2.000 |
| R因子 | 0.21631 |
| Rwork | 0.214 |
| R-free | 0.26153 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wao |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.015 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.684 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 30.000 | 2.056 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.038 | 0.435 |
| 独立反射数 | 34075 | |
| <I/σ(I)> | 28.8 | 5.6 |
| 完全性 [%] | 98.5 | 93.1 |
| 冗長性 | 8.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.2 M Mg(NO3)2, 20% PEG 3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
