4J1J

Leanyer orthobunyavirus nucleoprotein-ssDNA complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 1
格子定数 [Å]	50.403, 73.199, 77.905
格子定数 [度]	70.89, 82.54, 73.73

精密化法

残基	43.330 - 2.650
R因子	0.2236
Rwork	0.223
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j1g
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.282
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	43.330	2.780
分解能 [Å] (高)	2.650	2.680
独立反射数	27429
完全性 [%]	94.7	84

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.2 M ammonium sulfate, 0.1 M Tris-HCl (pH 7.5) and 20% w/v PEG5000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 289K