4IT6

Crystal structure of amino acid residues 1-120 of CG17282

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97243, 0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	87.503, 46.461, 69.146
Unit cell angles	90.00, 105.64, 90.00

Refinement procedure

Resolution	30.412 - 1.900
R-factor	0.2629
Rwork	0.261
R-free	0.27960
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.096
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.114	0.076	0.499
Number of reflections	20495
<I/σ(I)>	7.1
Completeness [%]	96.2	99.6	74.5
Redundancy	6.3	7.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	293	4M sodium formate pH 6.9, 0.1 M Na-cacodylate pH 6.5 and 1% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K