4IK1
High resolution structure of GCaMPJ at pH 8.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 119.939, 119.939, 98.616 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.928 - 2.000 |
R-factor | 0.1749 |
Rwork | 0.173 |
R-free | 0.20470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.718 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.930 | 1.990 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 49938 | |
Completeness [%] | 94.4 | 82 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | Tris pH 8.5, (NH4)2SO4, 25% PEG 3350, 0.5% DDAO, VAPOR DIFFUSION, HANGING DROP, temperature 289K |