4GVS

X-ray structure of the Archaeoglobus fulgidus methenyl-tetrahydromethanopterin cyclohydrolase in complex with N5-formyl-tetrahydromethanopterin

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Collection date	2010-04-28
Wavelength(s)	1.214
Spacegroup name	P 21 21 21
格子定数 [Å]	91.900, 108.990, 114.910
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	43.400 - 1.750
R因子	0.18902
Rwork	0.187
R-free	0.22421
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qlm
結合長の平均二乗偏差(RMSD) [Å]	0.019
結合角の平均二乗偏差(RMSD) [度]	1.971
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC (5.6.0117)

Quality characteristics

	Overall
分解能 [Å] (低)	43.402
分解能 [Å] (高)	1.750
独立反射数	108423

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	8% (w/v) PEG 4000, 0.1M ammonium acetate, 0.1M sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K