4GVR

X-ray structure of the Archaeoglobus fulgidus methenyl-tetrahydromethanopterin cyclohydrolase

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Collection date	2011-12-08
Wavelength(s)	1.0001
Spacegroup name	C 1 2 1
格子定数 [Å]	118.540, 122.080, 79.390
格子定数 [度]	90.00, 99.02, 90.00

精密化法

残基	48.170 - 1.520
R因子	0.16754
Rwork	0.164
R-free	0.23124
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qlm
結合長の平均二乗偏差(RMSD) [Å]	0.018
結合角の平均二乗偏差(RMSD) [度]	1.890
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC (5.6.0117)

Quality characteristics

	Overall
分解能 [Å] (低)	48.170
分解能 [Å] (高)	1.520
独立反射数	164493

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	10% (w/v) PEG 4000, 10% (v/v) Isopropanol, 0.1M Sodium acetate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K