4GST

REACTION COORDINATE MOTION IN AN SNAR REACTION CATALYZED BY GLUTATHIONE TRANSFERASE

Experimental procedure

Spacegroup name	C 1 2 1
格子定数 [Å]	88.240, 69.440, 81.280
格子定数 [度]	90.00, 106.01, 90.00

精密化法

残基	6.000 * - 1.900
R因子	0.18
結合長の平均二乗偏差(RMSD) [Å]	0.017
結合角の平均二乗偏差(RMSD) [度]	0.040 *
Refinement software	GPRLSA

Quality characteristics

	Overall
分解能 [Å] (高)	1.900 *
Rmerge_l_obs	0.066 *
独立反射数	36138 *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	11.3 (mg/ml)
2	1	drop	(9R,-10R)-9,10-dihydro-9-(S-glutathionyl)-10-hydroxyphenanthrene	3.5 (mM)
3	1	drop	beta-octylglucopyranoside	0.4 (%(w/v))
4	1	drop	ammonium sulfate	40 (%(w/v)sat)
5	1	drop		10 (mM)
6	1	reservoir	ammonium sulfate	60-74 (%(w/v)sat)
7	1	reservoir	beta-octylglucopyranoside	0.46 (%(w/v))
8	1	reservoir		10 (mM)