4GJH
Crystal Structure of the TRAF domain of TRAF5
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-06-06 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 71.052, 90.579, 117.216 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 33.073 - 2.805 |
| R因子 | 0.227 |
| Rwork | 0.224 |
| R-free | 0.27310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.156 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 2.850 |
| 分解能 [Å] (高) | 2.800 | 7.590 | 2.800 |
| 独立反射数 | 17854 | ||
| 完全性 [%] | 93.8 | 98 | 100 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1M Bis-Tris, 15%(w/v) PEG10000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
