4GBT
Structural characterization of H-1 Parvovirus: comparison of infectious virions to replication defective particles
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0895 |
Spacegroup name | P 1 21 1 |
格子定数 [Å] | 255.200, 350.100, 272.100 |
格子定数 [度] | 90.00, 90.12, 90.00 |
精密化法
残基 | 40.000 - 3.200 |
Rwork | 0.256 |
R-free | 0.25920 |
結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
結合角の平均二乗偏差(RMSD) [度] | 1.366 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.3) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 40.000 | 3.310 |
分解能 [Å] (高) | 3.200 | 3.200 |
独立反射数 | 479478 | |
<I/σ(I)> | 4.9 | 1.3 |
完全性 [%] | 61.3 | 58.3 |
冗長性 | 1.7 | 1.5 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 10 mM Tris-HCl, 150 mM NaCl, 8mM CaCl2.2H2O and 3% PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |