4G7Y
Crystal structure of voltage sensing domain of Ci-VSP with fragment antibody (R217E, 2.8 A)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-27 |
| Detector | Mar 300 CCD |
| Spacegroup name | P 65 2 2 |
| 格子定数 [Å] | 121.010, 121.010, 229.570 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 104.800 - 2.800 |
| R因子 | 0.20887 |
| Rwork | 0.206 |
| R-free | 0.26078 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.634 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 104.800 |
| 分解能 [Å] (高) | 2.800 |
| 独立反射数 | 24429 |
| 完全性 [%] | 100.0 |
| 冗長性 | 8.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1 M Bicine, 0.3 M MgCl2, 24% PEG 2000, 10% Glycerol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1 M Bicine, 0.3 M MgCl2, 24% PEG 2000, 10% Glycerol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
