4G5R
Structure of LGN GL4/Galphai3 complex
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 61 2 2 |
| 格子定数 [Å] | 209.550, 209.550, 237.211 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 48.044 - 3.481 |
| R因子 | 0.2113 |
| Rwork | 0.209 |
| R-free | 0.25280 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.004 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.884 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 3.560 |
| 分解能 [Å] (高) | 3.480 | 3.500 |
| Rmerge_l_obs | 0.110 | 0.757 |
| 独立反射数 | 39267 | |
| <I/σ(I)> | 14.7 | 1.8 |
| 冗長性 | 4.4 | 4.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.5M ammonium sulfate, 1.0M lithium sulfate monohydrate, 0.1M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
