4FVK
Structural and functional characterization of neuraminidase-like molecule N10 derived from bat influenza A virus
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 4 21 2 |
| 格子定数 [Å] | 122.496, 122.496, 110.504 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 41.026 - 2.198 |
| R因子 | 0.2093 |
| Rwork | 0.207 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nss |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.333 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.280 |
| 分解能 [Å] (高) | 2.198 | 2.200 |
| Rmerge_l_obs | 0.105 | 0.554 |
| 独立反射数 | 43333 | |
| <I/σ(I)> | 27.907 | 6 |
| 完全性 [%] | 99.9 | 100 |
| 冗長性 | 15.5 | 16.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 30%(w/v) PEG2000 MME, 0.1M sodium acetate, 0.2M ammonium sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
