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4F4V

Human Insulin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsLNLS BEAMLINE W01B-MX2
Synchrotron siteLNLS
BeamlineW01B-MX2
Temperature [K]100
Detector technologyCCD
Collection date2010-10-01
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1.458600
Spacegroup nameH 3
Unit cell lengths81.970, 81.970, 33.690
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution24.435 - 1.637
R-factor0.2118
Rwork0.209
R-free0.26380
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.026
RMSD bond angle2.054
Data reduction softwareMOSFLM
Data scaling softwareSCALA (3.2.25)
Phasing softwarePHASER (2.1.4)
Refinement softwareREFMAC (5.5.0102)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]24.43524.4351.730
High resolution limit [Å]1.6375.1801.637
Rmerge0.1510.0680.682
Total number of observations10083414
Number of reflections9124
<I/σ(I)>6.38.50.5
Completeness [%]87.889.694.7
Redundancy2.83.42.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.52932 uL 0.1 M sodium phosphate, pH 5.5, 10% w/v PEG6000 + 2 uL 100 U/mL human insulin (Humulin R, lot # A 505073), cryoprotectant: mother liquor + 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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數據於2024-03-27公開中

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