4EYY
Crystal Structure of the IcmR-IcmQ complex from Legionella pneumophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-01 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 68.948, 113.411, 49.465 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.750 - 2.400 |
R-factor | 0.222 |
Rwork | 0.217 |
R-free | 0.26200 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.721 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.754 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.208 | 0.341 |
Number of reflections | 15596 | |
<I/σ(I)> | 20.8 | 5.4 |
Completeness [%] | 98.0 | 99.1 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 294 | Protein concentration 8 mg/ml. Selenomethionine-incorporated protein for SAD phasing. 1:1 ratio of protein to 24% PEG 1500, 0.1 M sodium citrate pH 5.9, 1 mM DTT and 1% ethylene glycol. Cryoprotected in mother liquor + 30% ethylene glycol and frozen in liquid nitrogen, VAPOR DIFFUSION, HANGING DROP, temperature 294K | ||
2 |