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4EYY

Crystal Structure of the IcmR-IcmQ complex from Legionella pneumophila

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]100
Detector technologyCCD
Collection date2010-02-01
DetectorADSC QUANTUM 315
Spacegroup nameP 21 21 2
Unit cell lengths68.948, 113.411, 49.465
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution19.750 - 2.400
R-factor0.222
Rwork0.217
R-free0.26200
Structure solution methodSAD
RMSD bond length0.017
RMSD bond angle1.721
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareSHARP
Refinement softwarePHENIX ((phenix.refine: 1.7.3_928))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]19.7542.490
High resolution limit [Å]2.4002.400
Rmerge0.2080.341
Number of reflections15596
<I/σ(I)>20.85.4
Completeness [%]98.099.1
Redundancy7.47.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1294Protein concentration 8 mg/ml. Selenomethionine-incorporated protein for SAD phasing. 1:1 ratio of protein to 24% PEG 1500, 0.1 M sodium citrate pH 5.9, 1 mM DTT and 1% ethylene glycol. Cryoprotected in mother liquor + 30% ethylene glycol and frozen in liquid nitrogen, VAPOR DIFFUSION, HANGING DROP, temperature 294K
2

217705

數據於2024-03-27公開中

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