4ESV
A New Twist on the Translocation Mechanism of Helicases from the Structure of DnaB with its Substrates
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 2 2 21 |
| 格子定数 [Å] | 149.124, 180.321, 279.128 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 47.920 - 3.200 |
| R因子 | 0.245 |
| Rwork | 0.242 |
| R-free | 0.28900 |
| Structure solution method | SIRAS |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.506 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 50.000 | 3.420 |
| 分解能 [Å] (高) | 3.200 | 7.100 | 3.200 |
| Rmerge_l_obs | 0.115 | 0.044 | |
| 独立反射数 | 124032 | ||
| <I/σ(I)> | 6.5 | ||
| 完全性 [%] | 88.3 | 89.3 | 87.6 |
| 冗長性 | 3 | 3.1 | 2.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 4-7% PEG3350, 0.2 M calcium chloride, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 4-7% PEG3350, 0.2 M calcium chloride, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 4-7% PEG3350, 0.2 M calcium chloride, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
