4EFO
Crystal structure of the ubiquitin-like domain of human TBK1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 34.353, 47.785, 61.832 |
| 格子定数 [度] | 90.00, 93.54, 90.00 |
精密化法
| 残基 | 30.857 - 1.769 |
| R因子 | 0.1713 |
| Rwork | 0.170 |
| R-free | 0.20390 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.015 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.800 |
| 分解能 [Å] (高) | 1.769 | 1.770 |
| 独立反射数 | 19650 | |
| 完全性 [%] | 99.8 | 97 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 289 | 0.1M BIS-TRIS,0.2M NaCl,21% PEG 3350, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
