4E94
Crystal structure of MccF-like protein from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 108.323, 118.912, 51.737 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.668 - 1.651 |
R-factor | 0.1472 |
Rwork | 0.146 |
R-free | 0.17530 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.119 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.067 | |
Number of reflections | 80740 | |
<I/σ(I)> | 24 | |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 2.0 M Ammonium sulfate, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |