4DRB

The crystal structure of FANCM bound MHF complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
格子定数 [Å]	111.317, 70.025, 115.751
格子定数 [度]	90.00, 91.41, 90.00

精密化法

残基	49.670 - 2.634
R因子	0.2223
Rwork	0.221
R-free	0.25560
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.002
結合角の平均二乗偏差(RMSD) [度]	0.539
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.730
分解能 [Å] (高)	2.634	2.634
Rmerge_l_obs		0.459
独立反射数	52925
<I/σ(I)>		4.62
完全性 [%]	100.0	100
冗長性		6.2

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8.5	285	0.1M Tris-HCl, pH 8.5, 0.2 M (NH4)2SO4, 25% PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 285K