4DA4
Structure of mouse DNMT1 (731-1602) bound to hemimethylated CpG DNA
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Detector technology | CCD |
| Collection date | 2011-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 89.680, 152.042, 96.053 |
| 格子定数 [度] | 90.00, 94.54, 90.00 |
精密化法
| 残基 | 39.470 - 2.600 |
| R因子 | 0.198 |
| Rwork | 0.196 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.006 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.690 |
| 分解能 [Å] (高) | 2.600 | 2.600 |
| 独立反射数 | 77126 | |
| <I/σ(I)> | 12.4 | 1.9 |
| 完全性 [%] | 97.9 | 98 |
| 冗長性 | 2.7 | 2.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 3 mM TCEP, 0.1 M sodium citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
