4D6O
THE CRYSTAL STRUCTURE OF I-DMOI IN COMPLEX WITH ITS TARGET DNA AT 1H INCUBATION IN 5MM MG (STATE 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.888, 70.672, 107.143 |
| Unit cell angles | 90.00, 119.80, 90.00 |
Refinement procedure
| Resolution | 46.489 - 2.200 |
| R-factor | 0.1866 |
| Rwork | 0.186 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB DENTRY 2VS7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.159 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.490 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 70363 | |
| <I/σ(I)> | 13.1 | 3.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.5 |
