4CT7
CNOT9-CNOT1 complex with bound tryptophan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-18 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.031, 66.984, 72.653 |
| Unit cell angles | 90.00, 99.85, 90.00 |
Refinement procedure
| Resolution | 48.271 - 1.897 |
| R-factor | 0.1852 |
| Rwork | 0.183 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fv2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.270 | 2.110 |
| High resolution limit [Å] | 1.897 | 1.897 |
| Rmerge | 0.050 | 0.550 |
| Number of reflections | 54804 | |
| <I/σ(I)> | 15.6 | 2.3 |
| Completeness [%] | 99.4 | 96.1 |
| Redundancy | 4.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 20 % PEG 3350, 200 MM AMMONIUM SULFATE, 50 MM MES PH 6.0 |
