4CBO
Crystal structure of Complement Factor D mutant R202A after ensemble refinement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.140, 67.310, 133.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.332 - 1.800 |
| R-factor | 0.166 |
| Rwork | 0.163 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dsu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.789 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.ENSEMBLE_REFINEMENT: DEV_1259)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.140 | 0.510 |
| Number of reflections | 37211 | |
| <I/σ(I)> | 4.9 | 1.9 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15% PEG 6000, 50 MM MES/NAOH PH 6.0 |
