4BTV
Structure of PhaZ7 PHB depolymerase in complex with 3HB trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.337, 199.374, 44.424 |
| Unit cell angles | 90.00, 114.24, 90.00 |
Refinement procedure
| Resolution | 19.848 - 1.594 |
| R-factor | 0.1339 |
| Rwork | 0.133 |
| R-free | 0.15750 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.210 |
| Number of reflections | 83783 | |
| <I/σ(I)> | 20.3 | 4 |
| Completeness [%] | 96.3 | 84.1 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | HANGING DROP VAPOUR DIFFUSION. 0.1 M NAOAC (PH 5.0), 0.2 M NACL, 20% W/V PEG 6000 |
