4B4C
Crystal structure of the DNA-binding domain of human CHD1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.464, 62.464, 128.085 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.340 - 1.620 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.22570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BALBES |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | AutoPROC |
| Data scaling software | AP_SCALE |
| Phasing software | BALBES |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.090 | 1.710 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.040 | 0.510 |
| Number of reflections | 32880 | |
| <I/σ(I)> | 36.2 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.9 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2M AMMONIUM SULFATE, 0.1M CITRATE PH 3.5 |
