4B0S
Structure of the Deamidase-Depupylase Dop of the Prokaryotic Ubiquitin-like Modification Pathway in Complex with ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2010-07-01 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.360, 70.410, 93.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.902 - 2.850 |
R-factor | 0.1921 |
Rwork | 0.189 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.789 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (FOR MR) |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.060 | 0.430 |
Number of reflections | 10376 | |
<I/σ(I)> | 15.69 | 2.89 |
Completeness [%] | 98.4 | 98.4 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 100 MM HEPES-HCL PH 7.2 (298 K), 14 % (V/V) PEG-3350 |