4B0R
Structure of the Deamidase-Depupylase Dop of the Prokaryotic Ubiquitin-like Modification Pathway
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2010-07-01 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.550, 71.470, 96.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.353 - 2.600 |
R-factor | 0.1962 |
Rwork | 0.194 |
R-free | 0.23800 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.003 |
RMSD bond angle | 0.808 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.000 | 2.650 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.070 | 0.610 |
Number of reflections | 14518 | |
<I/σ(I)> | 15.59 | 2.01 |
Completeness [%] | 99.2 | 99.7 |
Redundancy | 3.6 | 3.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 100 MM HEPES-HCL PH 7.2, 14 % (V/V) PEG-3350, 298 K |