4AT1

STRUCTURAL CONSEQUENCES OF EFFECTOR BINDING TO THE T STATE OF ASPARTATE CARBAMOYLTRANSFERASE. CRYSTAL STRUCTURES OF THE UNLIGATED AND ATP-, AND CTP-COMPLEXED ENZYMES AT 2.6-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 3 2 1
格子定数 [Å]	122.000, 122.000, 142.000
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	10.000 - 2.600
R因子	0.17 *
Rwork	0.160
結合長の平均二乗偏差(RMSD) [Å]	0.014 *
結合角の平均二乗偏差(RMSD) [度]	3.400 *
Refinement software	X-PLOR

Quality characteristics

	Overall
分解能 [Å] (低)	10.000 *
分解能 [Å] (高)	2.600 *
Rmerge_l_obs	0.046 *
Total number of observations	74676 *
独立反射数	23826 *

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Microdialysis *	5.7 *

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	1	maleic acid	100 (mM)
2	1	1	Tris-HCl	10 (mM)
3	1	1	2-mercaptoethanol	1 (mM)
4	1	1	EDTA	0.2 (mM)
5	1	1		0.3 (mM)
7	1	2	protein	6 (mg/ml)