4AK0
Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-06 |
| Detector | MARRESEARCH |
| Spacegroup name | I 21 3 |
| 格子定数 [Å] | 77.800, 77.800, 77.800 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 55.050 - 2.280 |
| R因子 | 0.17263 |
| Rwork | 0.166 |
| R-free | 0.23165 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b2f |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.018 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.802 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0119) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 55.000 | 2.420 |
| 分解能 [Å] (高) | 2.280 | 2.280 |
| Rmerge_l_obs | 0.120 | 0.380 |
| 独立反射数 | 3406 | |
| <I/σ(I)> | 18.4 | 7 |
| 完全性 [%] | 91.9 | 100 |
| 冗長性 | 12.1 | 12.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 7.8 | pH 7.8 |
