4AAF
Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 49.170, 71.430, 173.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.144 - 2.500 |
R-factor | 0.2011 |
Rwork | 0.196 |
R-free | 0.24730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xe0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.215 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.430 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.090 | 0.470 |
Number of reflections | 21522 | |
<I/σ(I)> | 3.7 | 1.6 |
Completeness [%] | 98.3 | 98.2 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 35% METOH, 0.1M SODIUM CACODYLATE PH 6.5, |