4A4P
crystal structure of the Sec7 domain from human cytohesin1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-13 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.090, 80.280, 51.900 |
Unit cell angles | 90.00, 90.17, 90.00 |
Refinement procedure
Resolution | 32.030 - 2.000 |
R-factor | 0.1762 |
Rwork | 0.174 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r8m |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.200 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.170 |
Number of reflections | 79470 | |
<I/σ(I)> | 7.8 | 3.8 |
Completeness [%] | 99.4 | 100 |
Redundancy | 3.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | PEG 3350 12%, AMMONIUM ACETATE 0.4M. GLYCEROL CRYO-PROTECTANT., pH 7 |