4A4C

Structure of phosphoTyr371-c-Cbl-UbcH5B-ZAP-70 complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 65 2 2
格子定数 [Å]	75.285, 75.285, 459.660
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	29.325 - 2.704
R因子	0.2059
Rwork	0.203
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2ESK 1fbv
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	1.308
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	30.000	2.850
分解能 [Å] (高)	2.700	2.700
Rmerge_l_obs	0.060	0.640
独立反射数	22290
<I/σ(I)>	29.9	4.2
完全性 [%]	99.5	98.6
冗長性	16.8	13.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1			277	50 MM HEPES, PH 7.5, 0.2 M KCL AND 31-35% (V/V) PENTAERYTHRITOL PROPOXYLATE (5/4 PO/OH) AT 4 DEGREES CELSIUS.