4HI6
Crystal structure of H112W mutant of borna disease virus matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 158.066, 158.066, 103.714 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.490 - 2.200 |
| R-factor | 0.223 |
| Rwork | 0.219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f1j |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.354 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.073 | 0.482 |
| Number of reflections | 49036 | |
| <I/σ(I)> | 9 | 2.1 |
| Completeness [%] | 99.8 | 96 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MgCl2, 12% (w/v) PEG6000, 0.1 M N-(2-Acetamido)iminodiacetic acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
