3ZQQ
Crystal structure of the full-length small terminase from a SPP1-like bacteriophage
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-04 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| 格子定数 [Å] | 98.184, 98.184, 323.818 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 107.000 - 4.000 |
| R因子 | 0.1996 |
| Rwork | 0.200 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2CMP AND 3ZQP |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.002 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.660 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 107.000 | 4.230 |
| 分解能 [Å] (高) | 4.010 | 4.010 |
| Rmerge_l_obs | 0.080 | 0.190 |
| 独立反射数 | 3308 | |
| <I/σ(I)> | 6.6 | 4.1 |
| 完全性 [%] | 62.0 | 18 |
| 冗長性 | 8.5 | 8.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 0.2 M MGCL2, 8 % PEG 2000, 8 % PEG 550MME, 0.1 M TRIS PH 8.0 |
