3WOE
Crystal structure of P23-45 gp39 (6-109) bound to Thermus thermophilus RNA polymerase beta-flap domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-24 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 99.232, 99.232, 117.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.616 - 2.351 |
| R-factor | 0.1836 |
| Rwork | 0.181 |
| R-free | 0.22530 |
| Structure solution method | MRSAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.062 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.350 |
| Number of reflections | 24886 |
| <I/σ(I)> | 19.8 |
| Completeness [%] | 99.5 |
| Redundancy | 17.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 50mM Tris-HCl (pH 8.0), 12% PEG 8000, 8% 2-propanol, 3% 1,6-hexanediol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
