3WGX
Crystal structure of ERp46 Trx2 in a complex with Prx4 C-term
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.098, 36.406, 40.594 |
| Unit cell angles | 81.17, 87.47, 85.40 |
Refinement procedure
| Resolution | 26.785 - 0.920 |
| R-factor | 0.1405 |
| Rwork | 0.140 |
| R-free | 0.15800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1386)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.785 | 0.940 |
| High resolution limit [Å] | 0.920 | 0.920 |
| Number of reflections | 126561 | |
| Completeness [%] | 89.9 | 62.3 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 0.1 M MES, 20% PEG3350 , pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
