3W0R
Crystal structure of a thermostable mutant of aminoglycoside phosphotransferase APH(4)-Ia (N202A), ternary complex with AMP-PNP and hygromycin B
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-02-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 2 1 |
| 格子定数 [Å] | 69.967, 69.967, 123.428 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 50.000 - 2.300 |
| R因子 | 0.18349 |
| Rwork | 0.182 |
| R-free | 0.20910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.018 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.616 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 60.630 | 2.380 |
| 分解能 [Å] (高) | 2.300 | 2.300 |
| 独立反射数 | 16072 | |
| 完全性 [%] | 99.0 | 92.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 0.1M sodium chloride, 12% MPD, 0.1M sodium acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
