3VPQ
Crystal structure of Bombyx mori sigma-class glutathione transferase in complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 56.081, 56.081, 209.244 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.700 - 1.702 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vpt |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.533 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.592 | |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 22392 | |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES, pH7.5, containing 30% PEG 400, 20% 1,2-propanediol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
