3VEJ
Crystal structure of the Get5 carboxyl domain from S. cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-01-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.020, 46.340, 28.270 |
Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
Resolution | 24.839 - 1.230 |
R-factor | 0.1782 |
Rwork | 0.177 |
R-free | 0.20390 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.082 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.340 | 1.260 |
High resolution limit [Å] | 1.230 | 1.230 |
Rmerge | 0.058 | 0.609 |
Number of reflections | 18288 | |
<I/σ(I)> | 11.63 | 2.05 |
Completeness [%] | 97.9 | 94.6 |
Redundancy | 4.2 | 3.87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 3.4 M ammonium sulfate, 0.1 M sodium citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |