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3V1F

Crystal structure of de novo designed MID1-zinc H35E mutant

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 23-ID-B
Synchrotron siteAPS
Beamline23-ID-B
Temperature [K]100
Detector technologyCCD
Collection date2011-10-12
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)1.0000
Spacegroup nameP 21 21 21
Unit cell lengths37.241, 46.528, 62.277
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution26.345 - 1.151
R-factor0.1621
Rwork0.161
R-free0.18630
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1yzm
RMSD bond length0.010
RMSD bond angle1.244
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: 1.7.3_927))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]26.3451.160
High resolution limit [Å]1.1511.150
Rmerge0.0520.650
Number of reflections72577
<I/σ(I)>31.31.5
Completeness [%]97.877.1
Redundancy3.53.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP42931-2 microliters protein (20 mg/ml, 100 mM ammonium acetate buffer) mixed with 1 microliter crystallization buffer (0.1 M sodium acetate, pH 4.0, 15% PEG 600, 10% isopropanol, 5% glycerol), VAPOR DIFFUSION, HANGING DROP, temperature 293K

217705

数据于2024-03-27公开中

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