3UII

crystal structure of human Survivin in complex with H3(1-10) peptide

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
格子定数 [Å]	115.250, 71.256, 81.636
格子定数 [度]	90.00, 128.46, 90.00

精密化法

残基	29.272 - 2.600
R因子	0.2247
Rwork	0.222
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f3h
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.430
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.690
分解能 [Å] (高)	2.600	2.600
Rmerge_l_obs	0.086	0.529
独立反射数	16412
<I/σ(I)>	10.1	2
完全性 [%]	98.9	96.4
冗長性	4	3.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M ammonium citrate tribasic, pH 7.0, 12% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K