3UAG
UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE
Experimental procedure
実験手法 | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09 |
Detector | MARRESEARCH |
Spacegroup name | P 41 |
格子定数 [Å] | 65.240, 65.240, 134.410 |
格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
残基 | 8.000 - 1.770 |
Rwork | 0.178 |
R-free | 0.22200 |
Structure solution method | OTHER |
結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
結合角の平均二乗偏差(RMSD) [度] | 25.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.1) |
Quality characteristics
Overall | Outer shell | |
分解能 [Å] (低) | 65.900 | 1.830 |
分解能 [Å] (高) | 1.770 | 1.770 |
Rmerge_l_obs | 0.066 * | 0.204 * |
独立反射数 | 54268 | |
<I/σ(I)> | 25.6 | 8.6 |
完全性 [%] | 99.2 | 98.7 |
冗長性 | 4.6 | 4.4 |
結晶化条件
結晶ID | 方法 | pH | 温度 | 溶液条件 |
1 | Vapor diffusion * | 7.5 * | drop consists of equal volume of protein and reservoir solutions * |
文献の結晶化試薬
ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
1 | 1 | drop | protein | 9.8 (mg/ml) | |
2 | 1 | drop | UMA | 1 (mM) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | HEPES | 20 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 1.6-1.8 (M) | |
7 | 1 | reservoir | HEPES | 100 (mM) |