3U66
Crystal structure of T6SS SciP/TssL from Escherichia Coli Enteroaggregative 042
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Detector technology | CCD |
Collection date | 2011-08-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 51.610, 82.030, 96.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.680 - 2.630 |
R-factor | 0.2375 |
Rwork | 0.235 |
R-free | 0.25730 |
Structure solution method | SAD |
RMSD bond length | 0.000 |
RMSD bond angle | 0.000 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.700 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.056 | 0.600 |
Number of reflections | 11601 | |
<I/σ(I)> | 20.2 | 2.5 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 5.7 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.6 | 273 | mixing 300 nL protein at 23mg/mL with 100 nL 4.3 M NaCl, 0.1 M Bicine pH 10.6. , VAPOR DIFFUSION, SITTING DROP, temperature 273K |
1 | VAPOR DIFFUSION, SITTING DROP | 10.6 | 273 | mixing 300 nL protein at 23mg/mL with 100 nL 4.3 M NaCl, 0.1 M Bicine pH 10.6. , VAPOR DIFFUSION, SITTING DROP, temperature 273K |