3U0Z
Tetramerization dynamics of the C-terminus underlies isoform-specific cAMP-gating in HCN channels
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97238 |
| Spacegroup name | I 4 |
| 格子定数 [Å] | 97.670, 97.670, 113.200 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 43.780 - 2.900 |
| R因子 | 0.20812 |
| Rwork | 0.205 |
| R-free | 0.27497 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q43 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.016 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.708 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 43.780 | 3.060 |
| 分解能 [Å] (高) | 2.900 | 2.900 |
| Rmerge_l_obs | 0.120 | 0.485 |
| 独立反射数 | 11821 | |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 6.6 | 6.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION | 5 | 278 | 20-22% PEG 3350, 0.4M Sodium Acetate buffer (pH 5.0), VAPOR DIFFUSION, temperature 278K |
