3TOJ
Structure of the SPRY domain of human Ash2L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 115.289, 115.289, 105.780 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.304 - 2.070 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.20610 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.016 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.110 |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 49204 | |
Completeness [%] | 99.8 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 20% PEG4000, 0.15 M Li2SO4, 0.1 M Tris, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |