3TOJ
Structure of the SPRY domain of human Ash2L
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 115.289, 115.289, 105.780 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 36.304 - 2.070 |
| R因子 | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.20610 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.016 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 100.000 | 2.110 |
| 分解能 [Å] (高) | 2.070 | 2.070 |
| 独立反射数 | 49204 | |
| 完全性 [%] | 99.8 | 99 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 20% PEG4000, 0.15 M Li2SO4, 0.1 M Tris, pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
