3TKS
Crystal structure of full-length human peroxiredoxin 4 in three different redox states
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-08-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 183.178, 140.104, 61.504 |
| 格子定数 [度] | 90.00, 102.48, 90.00 |
精密化法
| 残基 | 30.523 - 2.400 |
| R因子 | 0.1858 |
| Rwork | 0.183 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pn8 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.113 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Quality characteristics
| Overall | Inner shell | |
| 分解能 [Å] (低) | 50.000 | 2.490 |
| 分解能 [Å] (高) | 2.400 | 2.400 |
| Rmerge_l_obs | 0.085 | 0.425 |
| 独立反射数 | 57443 | |
| <I/σ(I)> | 9.2 | |
| 完全性 [%] | 97.2 | 94 |
| 冗長性 | 3.1 | 2.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 290 | 0.8M sodium phosphate monobasic monohydrate/potassium phosphate dibasic, 5mM DTT, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
