3T2G
Fructose-1,6-bisphosphate aldolase/phosphatase from Thermoproteus neutrophilus, Y229F variant with DHAP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2010-08-01 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | I 4 2 2 |
| 格子定数 [Å] | 112.430, 112.430, 151.520 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 47.720 - 3.000 |
| R因子 | 0.1779 |
| Rwork | 0.175 |
| R-free | 0.23403 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t2b |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.600 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 47.721 | 3.160 |
| 分解能 [Å] (高) | 2.998 | 3.000 |
| Rmerge_l_obs | 0.180 | 0.599 |
| 独立反射数 | 10089 | |
| <I/σ(I)> | 10.7 | 3 |
| 完全性 [%] | 99.3 | 99.6 |
| 冗長性 | 6.4 | 6.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 8% PEG3350, 0.1 M HEPES/NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
